Activities of Solanum Marginatum L.Against Some Pathogenic Bacteria

Activities of Solanum Marginatum L.Against Some Pathogenic Bacteria

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Title: Activities of Solanum Marginatum L.Against Some Pathogenic Bacteria
Author: Fasil, Ahmed; Dr. Ameha, Kebede
Abstract: The emergence of antibiotic resistance is a public health problem of increasing magnitude. It is, therefore, important to look for more effective, safer and less toxic options of treatment. The aim of this study was to assess the antibacterial activities of crude extracts of different parts of Solanum marginatum against Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes and Campylobacter jejuni. The stems, fruits, roots and leaves of the plant were shade dried and ground to powder and the bioactive components were extracted using ethanol (99%) and water. The antibacterial activities of the extracts were evaluated using agar-well diffusion method and the inhibitory zones were recorded in millimeters. The minimum inhibitory concentration (MIC) of the plant extracts against E. coli, S. aureus, S. pyogenes and C. jejuni were assessed using the agar dilution method. The antibiotics Tetracycline, Amoxicillin and Penicillin were used as positive controls. The bioassay studies of the crude extracts were undertaken at two different concentrations (20 and 40 mg/ml).The results revealed that the crude extracts of ethanol had antibacterial activities against all bacterial strains. The ethanol extracts had growth inhibitory effect at a concentration of 20 mg/ml and 40 mg/ml with zones of inhibition ranging from 7.10-17.07 mm and 9.40-23.87 mm, respectively. The aqueous extracts showed some antibacterial activity at a concentration of 20 and 40 mg/ml with zones of inhibition ranging from 0.9 – 4.97 mm and 2.87-9.97mm, respectively. However, the two crude extracts had less antibacterial activities than Tetracycline. Campylobacter jejuni was found to be the most susceptible bacterium to crude ethanol extract of fruits and roots with minimum inhibitory concentration of 1.25 mg/ml and 2.5 mg/ml respectively, whereas Streptococcus pyogenes was the least susceptible bacterium to all of the crude extracts. The growth inhibitory activities of the crude extracts were found to be significantly different for the two concentrations (20 and 40 mg/ml) (p < 0.05). In conclusion, this study did not only show the antibacterial activities of Solanum marginatum, but also provided a scientific basis for its traditional use against some diseases.
Description: 76p.
URI: http://hdl.handle.net/123456789/3198
Date: 2015-10


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